Primers for the amplification and sequencing of the envelope of SIVmac251
Primer | Sequence | Description |
|---|---|---|
| KF92 | 5′-TCAACTATACCGCCCTCTTGAGGCGTGC-3′ | Outer sense primer for amplification of env |
| KF93 | 5′-TTGCACTGTAATAAATCCCTTCCAGTCCCC-3′ | Outer antisense primer for amplification of env |
| KF94 | 5′-TATGAGCAGTCACGAAAGAGAAGAAGAACT-3′ | Inner sense primer for amplification of region 1 |
| KF95 | 5′-CTTAGGGGAACTTTTGGCCTCACTGATACC-3′ | Inner antisense primer for amplification of region 1 |
| KF102 | 5′-AATTTGTCCACATGAAGGTAACTTCCG-3′ | Inner sense primer for amplification of region 2 |
| KF103 | 5′-AACCAATCAATGATAGGCCAAAGCAGGC-3′ | Inner antisense primer for amplification of region 2 |
| KF94 | 5′-TATGAGCAGTCACGAAAGAGAAGAAGAACT-3′ | Sense primer for sequencing of region 1 |
| KF67 | 5′-ACTTGGGGAACAACTCAGTGCC-3′ | Sense primer for sequencing of region 1 |
| KF68 | 5′-GAGACTAGTTCTTGTATAGC-3′ | Sense primer for sequencing of region 1 |
| KF69 | 5′-CAAGGATGATGGAGACACAGAC-3′ | Sense primer for sequencing of region 1 |
| KF70 | 5′-TAAATTGGGTAGARGATAGG-3′ | Sense primer for sequencing of region 2 |
| KF71 | 5′-TAGGGTTCTTGGGTTTTCTCG-3′ | Sense primer for sequencing of region 2 |
| KF72 | 5′-ATGTATGAATTACAAAAGTTG-3′ | Sense primer for sequencing of region 2 |
| KF73 | 5′-AGYATACCAGATCCTCCAACC-3′ | Sense primer for sequencing of region 2 |
| KF74 | 5′-TCCACCGTCTCRTTCTTTGCC-3′ | Antisense primer for sequencing of region 2 |
| KF75 | 5′-AACCTTTCGCTCCCACTCTTGCC-3′ | Antisense primer for sequencing of region 2 |
| KF76 | 5′-TGTACCTCTTCACATCTGTGGG-3′ | Antisense primer for sequencing of region 2 |
| KF77 | 5′-TTATCAGTATTGTTAGTTCC-3′ | Antisense primer for sequencing of region 1 |
| KF78 | 5′-AAAGCATAACCTGGAGGTGC-3′ | Antisense primer for sequencing of region 1 |
| KF79 | 5′-CCCCATCTATCTGTCTCACTT-3′ | Antisense primer for sequencing of region 1 |
| KF95 | 5′-CTTAGGGGAACTTTTGGCCTCACTGATACC-3′ | Antisense primer for sequencing of region 2 |