Mutations at the amino acid positions of the gp 120 envelope of SIVmac251
In most cases, solely the mutated nucleotide was detected. In a few cases, a mixture of mutant and wild-type nucleotide (roughly estimated to be at a ratio around 50/50) was observed and indicated by the designation of wild-type/mutant amino acid.
Glycosylated Asparagine Sites in SIV Env | Mutations in the Envelope That Emerged underEscalating Drug Exposure in MT4 Cell Cultures | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PRM-A-1 | PRM-A-2 | PRM-A-3 | PRM-A-4 | HHA-1 | HHA-2 | UDA-1 | UDA-2 | ||||||||
| 37NAT39 | |||||||||||||||
| 70NVT72 | 70Asn/Asp | ||||||||||||||
| 114NKS116 | |||||||||||||||
| 148NET150 | |||||||||||||||
| 158NCT160 | |||||||||||||||
| 173NMT175a | |||||||||||||||
| 186NET188 | |||||||||||||||
| 200NST202 | 200Asn/Ser | 200Asn/Asp | |||||||||||||
| 204NES206 | 204Asp | ||||||||||||||
| 214NTS216 | |||||||||||||||
| 246NDT248 | 248Ile | 248Ile | 248Pro | 248Ile | 248Ile | ||||||||||
| 249NYS251 | |||||||||||||||
| 280NGT282 | |||||||||||||||
| 286NRT288 | |||||||||||||||
| 297NRT299 | |||||||||||||||
| 308NLT310 | |||||||||||||||
| 318NKT320 | |||||||||||||||
| 373NNT375 | 375Ile | ||||||||||||||
| 379NLT381 | |||||||||||||||
| 462NST464 | 462Ser | 462Ser | 462Asp | 462Asp | 462Thr | 462Thr | 462Ser | 462Ser | |||||||
| 478NQT480 | |||||||||||||||
| 627NAS629 | |||||||||||||||
| 636NDT638 | 636Asn/Lys | ||||||||||||||
| 652NIT654 |
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↵ a In the SIVmac251 strain used in the selection experiments, an extra glycosylation site was created as a result of a T173N mutation.