PCR primers
Gene | Primer Sequence | Product Size | |
|---|---|---|---|
| bp | |||
| Occludin | 189 | ||
| Forward | TCAGGGAATATCCACCTATCACTTCAG | ||
| Reverse | CATCAGCAGCAGCCATGTACTCTTCAC | ||
| ZO-1 | 712 | ||
| Forward | CGGTCCTCTGAGCCTGTAAG | ||
| Reverse | GGATCTACATGCGACGACAA | ||
| ZO-2 | 220 | ||
| Forward | CGGATTCCAGACAAGGTGTT | ||
| Reverse | CCTTCAGAGACCCAGACTGC | ||
| Claudin-1 | 593 | ||
| Forward | AACGCGGGGCTGCAGCTGTTC | ||
| Reverse | GGATAGGGCCTTGGTCTTGGGT | ||
| Claudin-4 | 249 | ||
| Forward | AGCCTTCCAGGTCCTCAACT | ||
| Reverse | AGCAGCGAGTCGTACACCTT | ||
| Claudin-7 | 252 | ||
| Forward | AGGCATAATTTTCATCGTGG | ||
| Reverse | GAGTTGGACTTAGGGTAAGAGCG | ||
| GATA-1 | 377 | ||
| Forward | TCAATTCAGCAGCCTATTCC | ||
| Reverse | TTCGAGTCTGAATACCATCC | ||
| GATA-2 | 279 | ||
| Forward | TGTTGTGCAAATTGTCAGACG | ||
| Reverse | CATAGGTGCCATGTGTCCAGC | ||
| GATA-3 | 251 | ||
| Forward | AAGTGCATGACTCACTGGAGG | ||
| Reverse | TAGGCTTCATGATACTGCTCC | ||
| GATA-4 | 263 | ||
| Forward | CTGGCCTGTCATCTCACTACG | ||
| Reverse | GGTCCGTGCAGGAATTTGAGG | ||
| GATA-5 | 290 | ||
| Forward | TCGCCAGCACTGACAGCTCAG | ||
| Reverse | TGGTCTGTTCCAGGCTGTTCC | ||
| GATA-6 | 300 | ||
| Forward | TTCTAACTCAGATGATTGCAGC | ||
| Reverse | GCTGCACAAAAGCAGACACG | ||
| Glucose-3 phosphate dehydrogenase | 452 | ||
| Forward | ACCACAGTCCATGCCATCAC | ||
| Reverse | TCCACCACCCTGTTGCTGTA |
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