Effect of the most potent TNF-α inducers on Ca2+ mobilization, chemotaxis, and ROS production in human neutrophils and murine J774.A1 macrophages
Chemotactic responses are expressed as percentage of response induced by 50 nM IL-8 or 50 nM KC in human and murine neutrophils, respectively
Compound | Ca2+ Mobilization EC50 | Chemotaxis | ROS Production AC50 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Human PMN | Murine MΦ | Human PMN | Murine PMN | Human PMN | Murine MΦ | ||||
| μM | % | μM | |||||||
| 1 | 0.3 ± 0.2 | 24.1 ± 4.1 | 67.8 ± 9.4 | 68.9 ± 7.8 | 0.2 ± 0.02 | 25.4 ± 4.0 | |||
| 2 | 0.6 ± 0.4 | 2.3 ± 0.15 | 91.3 ± 1.6 | 58.0 ± 1.4 | 0.2 ± 0.04 | 8.6 ± 2.7 | |||
|
AG-14
| 0.63 ± 0.39 | 4.2 ± 0.8 | 98.0 ± 3.4 | 174.8 ± 9.2 | 38.6 ± 3.3 | 26.9 ± 3.9 | |||
MΦ, macrophages; PMN, polymorphonuclear neutrophils.