Desensitization of Ca2+ response induced in CHO/CXCR4 cells by synthetic intact versus truncated CXCL12
Results shown are the mean of two experiments, intracellular calcium responses were determined as described in Gouwy et al. (2004). Inhibition indicates the percentage inhibition of the second stimulus by the first stimulus.
First Stimulus | Second Stimulus | Inhibition | ||||
|---|---|---|---|---|---|---|
| Chemokine | Increase in [Ca2+]i | Chemokine | Increase in [Ca2+]i | |||
| nM | nM | % | ||||
| CXCL12(1-68) 10 ng/ml | 304 | 10 ng/ml CXCL12(1-68) | 63 | 79 | ||
| CXCL12(3-68) 100 ng/ml | 0 | 10 ng/ml CXCL12(1-68) | 323 | 0 | ||
| CXCL12(3-68) 300 ng/ml | 0 | 10 ng/ml CXCL12(1-68) | 368 | 0 | ||
| CXCL12(3-68) 1 μg/ml | 0 | 10 ng/ml CXCL12(1-68) | 166 | 45 | ||
| CXCL12(3-68) 3 μg/ml | 0 | 10 ng/ml CXCL12(1-68) | 72 | 76 | ||