Cell lines developed and used in this study
Cell Line Name and Clone No. | TMZ IC50 | Cell Line Description | Growth Media |
|---|---|---|---|
| mM | |||
| MDA-MB-231 | 2.0 | Human, Caucasian, breast, adenocarcinoma, epithelial cell | RPMI 1640 medium (450 ml), heat-inactivated fetal bovine serum (10%), gentamicin (10 μg/ml) |
| MDA-MB-231/Pol β-KD | |||
| 2 | 0.8 | Human breast cancer cells expressing Pol β lentiviral shRNA | Growth media supplemented with puromycin (0.5 μg/ml) |
| 10 | 0.8 | ||
| 18 | 0.7 | ||
| MDA-MB-231/MPG | |||
| 4 | 0.57 | MPG overexpression in human breast cancer cells | Growth media supplemented with G418 (800 μg/ml) |
| 5 | 0.50 | ||
| 6 | 0.90 | ||
| MDA-MB-231/Pol β-KD(10)/MPG | |||
| 1 | 0.27 | MPG overexpression in Pol β down-regulated human breast cancer cells | Growth media supplemented with puromycin (0.5 μg/ml) and G418 (750 μg/ml) |
| 2 | 0.27 | ||
| MDA-MB-231/Pol β-KD(10)/FLAG-Pol β-WT | |||
| 2 | 1.6 | WT FLAG-Pol β reconstituted in Pol β down-regulated human breast cancer cells | Growth media supplemented with puromycin (0.5 μg/ml) and G418 (700 μg/ml) |
| 3 | 2.0 | ||
| 4 | 1.5 | ||
| MDA-MB-231/Pol β-KD(10)/FLAG-Pol β-D25A | |||
| 3 | 1.63 | D256A mutant FLAG-Pol β reconstituted in Pol β down-regulated human breast cancer cells | Growth media supplemented with puromycin (0.5 μg/ml) and G418 (700 μg/ml) |
| 4 | 1.27 | ||
| 5 | 1.66 | ||
| MDA-MB-231/MPG(4)/FLAG-Pol β-WT | |||
| 19 | 1.31 | WT FLAG-Pol β reconstituted in MPG-overexpressing human breast cancer cells | Growth media supplemented with puromycin (0.5 μg/ml) and G418 (700 μg/ml) |
| 20 | 2.0 | ||
| 21 | 1.88 |
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