PAI-1 variant | Secondary Structural Element | Binding in Phage ELISA | Binding in BIACORE |
---|---|---|---|
fraction of control | |||
wt | 1.00 | 1.00 | |
Y81A | hD | 0.14 ± 0.05a | 0.25 ± 0.03a |
P87A | hD-s2Aloop | 0.23 ± 0.01a | 0.11 ± 0.01a |
W88A | hD-s2Aloop | 0.18 ± 0.07a | 0.01 ± 0.02a |
K90A | s2A | 0.36 ± 0.06a | 0.27 ± 0.00a |
D91A | s2A | 0.17 ± 0.06a | 0.11 ± 0.01a |
E92A | s2A | 0.17 ± 0.06a | 0.40 ± 0.14a |
Murine wt | N.D. | 0.15 ± 0.01a | |
Murine-hF-human chimera | N.D. | 0.39 ± 0.06a | |
Human-hF-murine chimera | N.D. | 0.06 ± 0.00a | |
wt, D1D2 chip | −0.01 ± 0.04a |
N.D., not determined, because the murine and murine-human chimeras react differently from the human protein with the antibodies employed in the ELISA.
↵ a Significantly different from wt binding to the paionin-4-D1D2 according to Student's t-test (P < 0.01).