Residue | CREB Activity | Expression | Relative CA | n | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mutation | Number | Schwartz | Ballest | Three Most Common AAs | 15 ng/well | 15 ng | 25 ng | ||||||||
% | -fold | ||||||||||||||
TM III | T(III:04)A | Thr107 | Thr III:04 | T3.28 | V, 19%; S, 10%; W, 10% | 97 ± 3.2 | 94 ± 1.6 | 1.0 ± 0.05 | 1.1 ± 0.07 | 3 | |||||
T(III:04)E | 76 ± 12 | 53 ± 11 | 1.4 ± 0.08 | 1.2 ± 0.06 | 4 | ||||||||||
F(III:08)A | Phe111 | Phe III:08 | F3.32 | D, 20%; F, 15%; L, 9% | 78 ± 10 | 108 ± 18 | 0.74 ± 0.05 | 0.82 ± 0.04 | 6 | ||||||
F(III:08)Y | 73 ± 11 | 39 ± 6.4 | 1.9 ± 0.11 | 1.8 ± 0.14 | 6 | ||||||||||
T(III:12)A | Thr115 | Thr III:12 | T3.36 | M, 20%; L, 15%; V, 11% | 116 ± 10 | 126 ± 31 | 0.93 ± 0.07 | 1.2 ± 0.09 | 3 | ||||||
T(III:12)F | 142 ± 41 | 31 ± 91 | 0.46 ± 0.01 | 0.68 ± 0.09 | 4 | ||||||||||
TM V | C(V:09)A | Cys201 | Cys V:09 | C5.43 | F, 23%; L, 13%; S, 10% | 45 ± 5.9 | 71 ± 7.5 | 0.63 ± 0.07 | 0.68 ± 0.07 | 4 | |||||
G(V:12)W | Gly204 | Gly V:12 | C5.46 | G, 29%; L, 14%; A, 11% | 40 ± 2.7 | 66 ± 5.2 | 0.61 ± 0.03 | 0.64 ± 0.04 | 4 | ||||||
Y(V:13)A | Tyr205 | Tyr V:13 | C5.47 | F, 70%; Y, 11%; L, 4% | 38 ± 4.7 | 54 ± 9.2 | 0.70 ± 0.08 | 0.64 ± 0.03 | 4 | ||||||
Y(V:13)F | 86 ± 7.3 | 61 ± 14 | 1.4 ± 0.13 | 1.4 ± 0.10 | 4 | ||||||||||
TM VI | F(VI:09)A | Phe253 | Phe VI:09 | F6.44 | F, 82%; Y, 6%; L, 3% | 75 ± 1.4 | 81 ± 8.9 | 0.91 ± 0.10 | 1.0 ± 0.07 | 5 | |||||
C(VI:12)A | Cys256 | Cys VI:12 | C6.47 | C, 74%; S, 9%; F, 5% | 95 ± 1.8 | 41 ± 1.6 | 2.3 ± 0.21a | 2.4 ± 0.19a | 4 | ||||||
F(VI:13)A | Phe257 | Phe VI:13 | F6.48 | W, 71%; F, 16%; Q, 2% | 39 ± 7.5 | 7.0 ± 1.1 | 5.7 ± 0.50b | 4.7 ± 0.27b | 5 | ||||||
F(VI:13)C | 54 ± 8.3 | 17 ± 1.8 | 3.1 ± 0.27a | 4.8 ± 0.27b | 6 | ||||||||||
F(VI:13)W | 67 ± 5.3 | 73 ± 11 | 0.9 ± 0.08 | 1.0 ± 0.13 | 4 | ||||||||||
Y(VI:16)A | Tyr260 | Tyr VI:16 | Y6.51 | Y, 36%; F, 30%; L, 12% | 25 ± 3.6 | 57 ± 7.3 | 0.43 ± 0.04 | 0.53 ± 0.07 | 6 | ||||||
Y(VI:16)F | 49 ± 4.7 | 96 ± 15 | 0.53 ± 0.03 | 0.59 ± 0.14 | 5 | ||||||||||
M(VI:24)A | Met268 | Met VI:24 | M6.59 | A, 16%; T, 14%; L, 12% | 89 ± 4.7 | 109 ± 9.2 | 0.82 ± 0.07 | 0.84 ± 0.07 | 5 | ||||||
TM VII | T(VII:05)A | Thr293 | Thr VII:05 | T7.38 | T, 24%; A, 15%; S, 13% | 99 ± 7.1 | 126 ± 8.0 | 0.87 ± 0.05 | 0.69 ± 0.13 | 6 | |||||
V(VII:06)A | Val294 | Val VII:06 | V7.39 | L, 21%; V, 11%; T, 11% | 110 ± 11 | 88 ± 5.9 | 1.3 ± 0.07 | 1.4 ± 0.10 | 4 | ||||||
M(VII:09)A | Met297 | Met VII:09 | M7.42 | A, 40%; G, 20%; S, 13% | 65 ± 2.3 | 93 ± 2.2 | 0.71 ± 0.02 | 0.78 ± 0.05 | 4 | ||||||
M(VII:09)F | 85 ± 15 | 80 ± 2.9 | 1.1 ± 0.06 | 1.4 ± 0.15 | 4 | ||||||||||
| N(VII:12)A | Asn300 | Asn VII:12 | N7.45 | N, 67%; S, 12%; H, 9% | 103 ± 5.8 | 146 ± 20 | 0.73 ± 0.08 | 0.81 ± 0.11 | 3 |