Gene & Gene Primer | Sequence | Size |
---|---|---|
bp | ||
RT-PCR | ||
Nurr1 | 297 | |
Forward | 5′-CGACATTTCTGCCTTCTCC-3′ | |
Reverse | 5′-GGTAAAGTGTCCAGGAAAAG-3′ | |
NOR-1 | 365 | |
Forward | 5′-TCTGCCTTCCAAACCAAAG-3′ | |
Reverse | 5′-TGATGGAAAGTCTGAGGAC-3′ | |
Nur77 | 748 | |
Forward | 5′-CGACCCCCTGACCCCTGAGTT-3′ | |
Reverse | 5′-GCCCTCAAGGTGTTGGAGAAGT-3′ | |
GAPDH | 965 | |
Forward | 5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′ | |
Reverse | 5′-CATGTGGGCCATGAGGTCCACCAC-3′ | |
Construction of plasmids | ||
pNur77-496/+67 | 564 | |
Forward | 5′-CGACGCGTGGCTTGGGAAGGTGTAAAGGC-3′ | |
Reverse | 5′-GGAAGATCTCCCAAGTTTCCGTAGCCAC-3′ | |
pNur77 mut | 564 | |
Forward | 5′-TCCCTGCGTTGATGGAACCCCGCGTGCGTTGCGCGCGCAGA-3′ | |
Reverse | 5′-TCTGCGCGCGCAACGCACGCGGGGTTCCATCAACGCAGG GAG-3′ |
|
bp, base pairs.