rAmy | hβCGRP | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mutant | pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | ||||||||||
WT | Mutant | WT | Mutant | WT | Mutant | WT | Mutant | |||||||
E35A AMY3(a) | 10.3 ± 0.16 (3) | 9.85 ± 0.25 (3) | 83.6 ± 1.63 (3) | 77.2 ± 5.02 (3) | 9.36 ± 0.14 (4) | 9.16 ± 0.28 (4) | 89.3 ± 5.77 (4) | 74.5 ± 6.57 (4) | ||||||
D46V AMY3(a) | 10.3 ± 0.16 (3) | 10.3 ± 0.22 (3) | 85.8 ± 3.38 (3) | 73.7 ± 6.75 (3) | 9.36 ± 0.14 (4) | 9.37 ± 0.23 (4) | 89.3 ± 5.77 (4) | 79.5 ± 5.51 (4) | ||||||
E74W AMY3(a) | 10.7 ± 0.08 (4) | 9.87 ± 0.09*** (4) | 88.1 ± 2.13 (4) | 76.8 ± 5.54 (4) | 10.1 ± 0.11 (3) | 9.60 ± 0.14* (3) | 85.1 ± 1.27 (3) | 81.8 ± 2.60 (3) | ||||||
P87A AMY3(a) | 10.6 ± 0.14 (4) | 10.2 ± 0.12 (4) | 93.0 ± 4.41 (4) | 79.2 ± 8.41 (4) | 10.1 ± 0.11 (3) | 9.83 ± 0.21 (3) | 85.6 ± 0.88 (3) | 80.4 ± 4.92 (3) | ||||||
L88E AMY3(a) | 10.7 ± 0.13 (3) | 10.2 ± 0.20 (3) | 95.2 ± 5.44 (3) | 84.6 ± 6.76 (3) | 10.1 ± 0.10 (3) | 9.73 ± 0.12 (3) | 86.0 ± 1.32 (3) | 79.4 ± 1.93* (3) | ||||||
A89V AMY3(a) | 10.7 ± 0.13 (3) | 10.5 ± 0.02 (3) | 95.2 ± 5.44 (3) | 86.1 ± 6.78 (3) | 10.0 ± 0.11 (3) | 10.2 ± 0.13 (3) | 85.3 ± 1.73 (3) | 85.9 ± 4.68 (3) | ||||||
I93F AMY3(a) | 10.7 ± 0.17 (4) | 10.1 ± 0.07* (4) | 89.3 ± 1.77 (4) | 84.6 ± 2.20 (4) | 10.0 ± 0.07 (4) | 9.14 ± 0.13** (4) | 84.9 ± 2.02 (4) | 75.5 ± 3.38 (4) | ||||||
N103S AMY3(a) | 10.8 ± 0.15 (3) | 9.69 ± 0.08** (3) | 87.2 ± 1.16 (3) | 80.2 ± 5.23 (3) | 10.1 ± 0.07 (3) | 9.06 ± 0.02*** (3) | 86.0 ± 2.88 (3) | 75.5 ± 1.72* (3) |