Summary of cAMP data for human mycRAMP1 mutants containing individual RAMP3 residues expressed with HA-CL
Emax values are cAMP percentage of forskolin. *p < 0.05, **p < 0.01 versus WT by unpaired t test.
Mutant | hAM | hαCGRP | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | |||||||||||
| WT | Mutant | WT | Mutant | WT | Mutant | WT | Mutant | |||||||
| A34E CGRP1 | 8.27 ± 0.19 (3) | 8.21 ± 0.07 (3) | 68.7 ± 5.84 (3) | 71.6 ± 0.85 (3) | 10.2 ± 0.07 (3) | 10.6 ± 0.07* (3) | 74.5 ± 0.27 (3) | 80.3 ± 0.96** (3) | ||||||
| V46D CGRP1 | 8.50 ± 0.16 (3) | 8.32 ± 0.10 (3) | 77.0 ± 1.48 (3) | 74.8 ± 2.78 (3) | 10.2 ± 0.11 (3) | 10.6 ± 0.13 (3) | 78.7 ± 3.61 (3) | 85.5 ± 4.13 (3) | ||||||
| W74E CGRP1 | 8.43 ± 0.15 (5) | 9.26 ± 0.08** (5) | 70.4 ± 2.65 (5) | 74.9 ± 3.20 (5) | 10.5 ± 0.20 (5) | 10.6 ± 0.20 (5) | 75.8 ± 3.88 (5) | 77.4 ± 3.75 (5) | ||||||
| A87P CGRP1 | 8.50 ± 0.15 (3) | 8.41 ± 0.09 (3) | 74.7 ± 1.17 (3) | 76.3 ± 2.85 (3) | 10.2 ± 0.16 (3) | 10.6 ± 0.25 (3) | 80.3 ± 2.79 (3) | 85.7 ± 5.13 (3) | ||||||
| E88L CGRP1 | 8.68 ± 0.16 (3) | 8.48 ± 0.06 (3) | 70.1 ± 5.29 (3) | 72.1 ± 3.68 (3) | 10.3 ± 0.14 (3) | 10.3 ± 0.29 (3) | 79.4 ± 2.31 (3) | 75.6 ± 0.85 (3) | ||||||
| V89A CGRP1 | 7.81 ± 0.43 (3) | 7.70 ± 0.15 (3) | 72.4 ± 3.25 (3) | 76.0 ± 3.02 (3) | 9.86 ± 0.18 (3) | 9.62 ± 0.19 (3) | 73.1 ± 5.58 (3) | 75.5 ± 1.93 (3) | ||||||
| F93I CGRP1 | 8.03 ± 0.25 (3) | 7.60 ± 0.18 (3) | 70.4 ± 2.08 (3) | 73.1 ± 4.35 (3) | 10.1 ± 0.15 (3) | 9.40 ± 0.12* (3) | 72.2 ± 4.79 (3) | 66.9 ± 4.98 (3) | ||||||
| S103N CGRP1 | 8.23 ± 0.29 (3) | 8.20 ± 0.16 (3) | 69.8 ± 3.33 (3) | 69.9 ± 2.20 (3) | 10.1 ± 0.25 (3) | 10.5 ± 0.16 (3) | 79.3 ± 3.43 (3) | 73.9 ± 3.78 (3) | ||||||