Summary of cAMP data for human mycRAMP1 mutants containing individual RAMP3 residues expressed with HA-CT(a)
Emax values are cAMP percentage of forskolin. *p < 0.05, **p < 0.01, ***p < 0.001 versus WT by unpaired t test. Values for HA-CT(a) alone: for rAmy, pEC50 ± S.E.M.: 8.85 ± 0.09 (n = 6) and Emax ± S.E.M.: 89.4 ± 2.05 (n = 6); for hβCGRP, pEC50 ± S.E.M.: 8.25 ± 0.04 (n = 8) and Emax ± S.E.M.: 80.9 ± 4.64 (n = 8).
rAmy | hβCGRP | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mutant | pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | pEC50 ± S.E.M. (n) | Emax ± S.E.M. (n) | ||||||||||
| WT | Mutant | WT | Mutant | WT | Mutant | WT | Mutant | |||||||
| A34E AMY1(a) | 10.4 ± 0.12 (3) | 10.8 ± 0.17 (3) | 75.5 ± 4.52 (3) | 80.1 ± 1.31 (3) | 10.7 ± 0.22 (3) | 10.9 ± 0.18 (3) | 71.6 ± 9.67 (3) | 79.1 ± 2.77 (3) | ||||||
| V46D AMY1(a) | 10.5 ± 0.11 (3) | 10.8 ± 0.13 (3) | 74.0 ± 0.81 (3) | 73.9 ± 7.21 (3) | 10.5 ± 0.11 (3) | 10.8 ± 0.12 (3) | 68.9 ± 7.43 (3) | 72.7 ± 3.95 (3) | ||||||
| W74E AMY1(a) | 10.2 ± 0.15 (4) | 10.9 ± 0.07* (4) | 77.7 ± 3.36 (4) | 73.1 ± 2.71 (4) | 10.7 ± 0.15 (3) | 11.0 ± 0.18 (3) | 83.0 ± 5.20 (3) | 80.0 ± 3.41 (3) | ||||||
| A87P AMY1(a) | 10.2 ± 0.16 (3) | 10.8 ± 0.16 (3) | 75.6 ± 3.76 (3) | 81.2 ± 3.51 (3) | 10.9 ± 0.04 (3) | 11.1 ± 0.09 (3) | 69.1 ± 6.94 (3) | 73.2 ± 4.65 (3) | ||||||
| E88L AMY1(a) | 10.2 ± 0.13 (3) | 9.96 ± 0.18 (3) | 69.8 ± 9.61 (3) | 73.3 ± 3.68 (3) | 10.9 ± 0.03 (3) | 10.4 ± 0.15* (3) | 79.8 ± 4.13 (3) | 80.8 ± 3.40 (3) | ||||||
| V89A AMY1(a) | 10.2 ± 0.15 (3) | 9.71 ± 0.02* (3) | 63.7 ± 6.69 (3) | 76.9 ± 12.41 (3) | 10.7 ± 0.13 (3) | 9.86 ± 0.09** (3) | 68.8 ± 2.48 (3) | 82.3 ± 5.62 (3) | ||||||
| F93I AMY1(a) | 10.3 ± 0.28 (3) | 9.08 ± 0.28* (3) | 74.9 ± 2.35 (3) | 79.8 ± 4.67 (3) | 10.7 ± 0.16 (3) | 9.04 ± 0.08*** (3) | 69.3 ± 7.26 (3) | 68.7 ± 2.35 (3) | ||||||
| S103N AMY1(a) | 10.5 ± 0.11 (3) | 10.9 ± 0.02* (3) | 73.8 ± 3.40 (3) | 79.9 ± 5.49 (3) | 10.7 ± 0.08 (3) | 10.9 ± 0.10 (3) | 73.4 ± 6.83 (3) | 76.3 ± 3.06 (3) | ||||||