Effect of different ligands on the transcriptional activity of LexA-PPARβ/δ fusion proteins in luciferase reporter assays
Values represent averages of three o eight independent experiments (± S.D.). Asterisks indicate statistically significant induction (*P < 0.05) or lack of significance (**P > 0.05) by t test. Data from different experiments were standardized to GW501516.
Compound | Properties | Concentration | -Fold Induction |
|---|---|---|---|
| μM | |||
| GW501516 | Synthetic PPARβ/δ agonist | 1.0 | 16.6 ± 6.0* |
| L165,041 | Synthetic PPAR/β/δ agonist | 2.0 | 11.2 ± 4.9* |
| cPGI | Synthetic PPARβ/δ agonist | 1.0 | 6.7 ± 1.7* |
| GW1929 | Synthetic PPARγ agonist | 0.3 | 1.3 ± 0.6** |
| Arachidonic acid | Pan-PPAR ligand | 20 | 2.6 ± 1.1* |
| Linoleic acid | Pan-PPAR ligand | 20 | 1.6 ± 0.3* |
| Eicosapentaenoic acid | Pan-PPAR ligand | 20 | 1.9 ± 0.4* |
| atRA | RAR agonist | 1.0 | 1.1 ± 0.1** |
| 9-cis-RA | RAR and RxR agonist | 1.0 | 1.0 ± 0** |