TABLE 2

Flow cytometry analysis of annexin V/propidium iodide in HaCaT cells after atRA or 9-cis RA

HaCaT cells were treated in triplicate for the indicated times with the indicated concentration of RA in the presence (top) or absence (bottom) of culture medium serum. Values (mean ± S.E.M.) with different letters in each column are significantly different, P < 0.05, as determined by ANOVA and Bonferroni's multiple comparison test.



48 h

72 h
Treatment
Early Apoptosis
Late Apoptosis
Early Apoptosis
Late Apoptosis
%
+ Serum
Vehicle 6.1 ± 0.5a 1.7 ± 0.3a 4.7 ± 0.3a 1.8 ± 0.7a
0.1 μM atRA 8.5 ± 0.8a 2.4 ± 0.6a 4.4 ± 0.3a 1.9 ± 0.5a
1.0 μM atRA 11.6 ± 0.7b 5.6 ± 0.9b 4.1 ± 0.3a 4.1 ± 0.1b
0.1 μM 9-cis RA 10.7 ± 1.0b 3.8 ± 1.1a 5.2 ± 0.4a 2.6 ± 0.7a
1.0 μM 9-cis RA 9.2 ± 1.0b 4.9 ± 1.4b 4.4 ± 0.1a 6.7 ± 1.0a
− Serum
Vehicle 0.6 ± 0.1a 1.0 ± 0.1a 1.0 ± 0.0a 0.4 ± 0.1a
0.1 μM atRA 0.5 ± 0.1a 0.6 ± 0.0a 0.8 ± 0.1a 0.2 ± 0.0a
1.0 μM atRA 0.4 ± 0.1a 1.0 ± 0.1a 1.0 ± 0.1a 0.6 ± 0.4a
0.1 μM 9-cis RA 0.4 ± 0.1a 1.0 ± 0.2a 0.9 ± 0.1a 0.2 ± 0.0a
1.0 μM 9-cis RA
0.4 ± 0.1a
1.1 ± 0.3a
1.1 ± 0.1a
0.4 ± 0.1a