Primers used to amplify Rxt1/NTT4 in RT-PCR
Position | Primer Sequence | Expected Size |
|---|---|---|
| bp | ||
| 475-2658 | 2184 | |
| Forward | 5′-ATGCCGAAGAACAGCAAGGTG-3′ | |
| Reverse | 5′-TCACAGCTCTGACTCAGGGGT-3′ | |
| 475-2648 | 2174 | |
| Forward | 5′-ATGCCGAAGAACAGCAAGGTG-3′ | |
| Reverse | 5′-GACTCAGGGGTGCTGGCCAAG-3′ | |
| 475-2112 | 2174 | |
| Forward | 5′-ATGCCGAAGAACAGCAAGGTG-3′ | |
| Reverse | 5′-GACTCAGGGGTGCTGGCCAAG-3′ | |
| 475-2112 | 1638 | |
| Forward | 5′-ATGCCGAAGAACAGCAAGGTG-3′ | |
| Reverse | 5′-AATCCAGGCCACAGCGATGTT-3′ | |
| 718-2112 | 1395 | |
| Forward | 5′-AACATCTGGAGGTTCCCCTAC-3′ | |
| Reverse | 5′-AATCCAGGCCACAGCGATGTT-3′ | |
| 2401-2658 | 258 | |
| Forward | 5′-TTGCTCTCCGACGGGTCCAAC-3′ | |
| Reverse | 5′-TCACAGCTCTGACTCAGGGGT-3′ | |
| 475-678 | 204 | |
| Forward | 5′-ATGCCGAAGAACAGCAAGGTG-3′ | |
| Reverse | 5′-CTGCAGCTTGCTATTCCAGGC-3′ |
|
bp, base pairs.