Results of fitting the inhibition data to a two-site, two-drug model
The results of fitting complete data sets (antagonists 1 and 2 separately and the pair of antagonists) to a two-site, two-drug inhibition model (eq. 3). With metocurine, there was no convergence for Lαδ; fitting process was halted when Lαδ = 30,000 nM. In all other cases, after convergence was reached, the Lαϵ and Lαδ values of antagonist 2 were reversed and used as seeds for a second fit. The results were the same as for the initial fit.
Antagonist 1 | Lαϵ (95% CL) | Lαδ (95% CL) | Antagonist 2 | Lαϵ (95% CL) | Lαδ (95% CL) | ||
|---|---|---|---|---|---|---|---|
| nM | nM | ||||||
| (+)-Tubocurarine | 31 (3) | 7700 (44000) | Metocurine | 58 (7) | |||
| 31 (2) | 5900 (30000) | Cisatracurium | 85 (20) | 250 (110) | |||
| 32 (3) | 2500 (7500) | Pancuronium | 170 (120) | 18 (3) | |||
| 32 (3) | 2600 (7500) | Vecuronium | 120 (80) | 29 (7) | |||
| 31 (4) | 3800 (19000) | Rocuronium | 82 (58) | 39 (16) | |||
| Metocurine | 60 (5) | Cisatracurium | 81 (17) | 280 (120) | |||
| 60 (8) | Pancuronium | 200 (200) | 18 (3) | ||||
| 59 (8) | Rocuronium | 99 (84) | 34 (13) | ||||
| Cisatracurium | 70 (14) | 460 (320) | Rocuronium | 54 (58) | 55 (57) | ||
| Pancuronium | 300 (380) | 17 (3) | Rocuronium | 35 (13) | 110 (100) | ||
| 380 (350) | 16 (2) | Cisatracurium | 69 (9) | 490 (240) | |||
| Vecuronium | 800 (1000) | 22 (2) | Pancuronium | 610 (910) | 16 (2) | ||
| 500 (770) | 23 (4) | Rocuronium | 59 (42) | 52 (37) | |||
|
| 520 (490) | 22 (2) | Cisatracurium | 70 (9) | 440 (180) | ||