TABLE 2

Expression of FGF and FGFR mRNAs in primary NSCLC tumors

Primary lung tumor total RNA obtained through the Tissue Procurement Core of the UCHSC Lung SPORE was reverse-transcribed and submitted to quantitative RT-PCR with primers (see Table 1) specific for FGF2, FGF9, FGFR1, or FGFR2. The values are normalized to GAPDH mRNA and presented as mean relative expression and S.E.M. The status of the FGFR IIIb/IIIc splice variants for FGFR1 and FGFR2 was determined as described in the legend to Fig. 4. FGFR1 IIIc was the sole FGFR1 isoform detected in the primary tumor mRNA samples.


Tumor ID

Relative Expression
FGF2
FGF9
FGFR1
FGFR2
FGFR2 isoform
11898 0.06 ± 0.001 0.04 ± 0.018 0.12 ± 0.027 0.29 ± 0.037 FGFR2b
18187 1.12 ± 0.24 0.00 ± 0.000 0.32 ± 0.073 0.56 ± 0.038 FGFR2c
16219 0.14 ± 0.000 0.46 ± 0.089 0.39 ± 0.115 0.07 ± 0.014
21023 1.00 ± 0.000 0.00 ± 0.001 0.49 ± 0.051 1.00 ± 0.000 FGFR2c
16895 0.13 ± 0.010 0.13 ± 0.055 0.18 ± 0.035 0.04 ± 0.001
21236 0.06 ± 0.025 1.00 ± 0.000 0.31 ± 0.045 0.51 ± 0.127 FGFR2b and c
21211 0.05 ± 0.002 0.01 ± 0.003 1.00 ± 0.000 0.03 ± 0.010
21460 0.04 ± 0.027 0.01 ± 0.005 0.10 ± 0.040 0.03 ± 0.004
21450
0.16 ± 0.018
0.03 ± 0.011
0.40 ± 0.030
0.46 ± 0.088
FGFR2b and c