B&W | Expression Level | n | Constitutive Activity | n | Ghrelin | n | Fmut | |
---|---|---|---|---|---|---|---|---|
% | nM | |||||||
WT-Ghrelin R1a | 1a | 3 | 46 ± 1 | 52 | 0.34 ± 0.02 | 52 | 1.0 | |
AspII:20Asn (Asp99) | 2.60 | 0.62 ± 0.15 | 3 | 69 ± 2 | 8 | 1.10 ± 0.2 | 8 | 3.4 |
PheIII:04Ser(Phe119) | 3.28 | 1.20 ± 0.21 | 3 | 39 ± 2 | 21 | 0.44 ± 0.04 | 17 | 1.3 |
GlnIII:05Ala(Gln120) | 3.29 | 0.37 ± 0.11 | 3 | 49 ± 3 | 11 | 2.30 ± 0.6 | 11 | 6.8 |
SerIII:08Ala (Ser123) | 3.32 | 1.20 ± 0.08 | 3 | 36 ± 2 | 16 | 0.77 ± 0.30 | 16 | 2.3 |
GluIII:09Gln (Glu124) | 3.33 | 0.75 ± 0.18 | 3 | 44 ± 2 | 10 | 52.00 ± 11 | 10 | 150 |
ThrIII:12Ala (Thr127)b | 3.36 | 0.73 ± 0.17 | 3 | 64 ± 5 | 4 | 0.61 ± 0.22 | 4 | 3.2 |
SerIV:16Ala (Ser174) | 4.56 | 0.78 ± 0.12 | 3 | 41 ± 3 | 10 | 0.65 ± 0.10 | 10 | 2.1 |
IleIV:20Ala (Ile178) | 4.60 | 1.00 ± 0.3 | 3 | 46 ± 2 | 10 | 0.82 ± 0.15 | 10 | 2.4 |
Arg199Leub | 0.94 ± 0.08 | 3 | 31 ± 3 | 7 | 0.76 ± 0.34 | 7 | 2.2 | |
Glu197Glnb | 0.99 ± 0.04 | 3 | 49 ± 2 | 8 | 2.30 ± 0.9 | 8 | 6.9 | |
MetV:05Ala (Met213) | 5.39 | 1.30 ± 0.4 | 3 | 49 ± 2 | 13 | 0.44 ± 0.07 | 13 | 1.3 |
ValV:08Ala (Val216) | 5.42 | 0.53 ± 0.06 | 3 | 53 ± 1 | 11 | 0.91 ± 0.24 | 6 | 2.7 |
SerV:09Ala (Ser217) | 5.43 | 0.73 ± 0.12 | 3 | 41 ± 2 | 7 | 0.37 ± 0.09 | 7 | 1.1 |
PheV:12Ala (Phe220) | 5.46 | 0.68 ± 0.07 | 3 | 22 ± 2 | 12 | 0.48 ± 0.08 | 13 | 1.4 |
PheVI:16Ala (Phe279) | 6.51 | 0.72 ± 0.23 | 3 | 0 ± 1 | 13 | 12.00 ± 3 | 12 | 36 |
ArgVI20:Gln (Arg283) | 6.55 | 0.67 ± 0.26 | 3 | 20 ± 2 | 12 | 19.00 ± 4 | 10 | 55 |
PheVI:23Ala (Phe286)b | 6.58 | 1.00 ± 0.20 | 45 ± 2 | 7 | 8.50 ± 2.6 | 7 | 25 | |
SerVI:24Ala (Ser287)b | 6.59 | 1.10 ± 0.20 | 3 | 50 ± 2 | 7 | 0.57 ± 0.16 | 7 | 1.7 |
GlnVII:-02Ala (Gln302)b | 7.32 | 0.49 ± 0.06 | 3 | 43 ± 4 | 6 | 1.90 ± 0.7 | 6 | 5.6 |
AsnVII:02Ala (Asn305) | 7.35 | 1.10 ± 0.2 | 3 | 26 ± 2 | 14 | 1.20 ± 0.3 | 11 | 3.5 |
PheVII:06Leu (Phe309) | 7.39 | 1.20 ± 0.5 | 3 | 38 ± 3 | 8 | 0.32 ± 0.07 | 8 | 0.9 |
PheVII:09Ala (Phe312) | 7.42 | 0.64 ± 0.14 | 3 | 24 ± 5 | 8 | 0.88 ± 0.13 | 6 | 2.6 |
Ξ-35 N-terminal |
| 0.28 ± 0.18 | 3 | 41 ± 4 | 5 | 0.67 ± 0.05 | 5 | 2.0 |