Effects on ifenprodil and zinc sensitivity of various mutations in NR2B NTD
NR2B Mutants | Ifenprodil | Zinc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IC50 | Mutant/wt Ratio | n | IC50 | Mutant/wt Ratio | n | |||||
| μM | μM | |||||||||
| wt | 0.16 ± 0.01 | 9 | 0.70 ± 0.08 | 15 | ||||||
| T76 | ||||||||||
| T76A | 2.3 ± 0.8 | 15 | 3 | 0.60 ± 0.06 | 0.8 | 6 | ||||
| T76C | 2.5 ± 0.1 | 16 | 3 | 0.9 ± 0.1 | 1.3 | 3 | ||||
| T76S | 0.43 ± 0.01 | 2.8 | 3 | 0.40 ± 0.05 | 0.6 | 3 | ||||
| D77 | ||||||||||
| D77C | 2.8 ± 0.2 | 18 | 4 | 0.9 ± 0.3 | 1.3 | 5 | ||||
| Q153 | ||||||||||
| Q153A | 0.22 ± 0.04 | 1.4 | 7 | 0.8 ± 0.3 | 1.1 | 4 | ||||
| Q153C | 0.28 ± 0.02 | 1.8 | 7 | 0.8 ± 0.3 | 1.1 | 6 | ||||
| D206 | ||||||||||
| D206A | 4 ± 1 | 30 | 7 | 2.6 ± 0.4 | 3.7 | 6 | ||||
| D206C | 5 ± 2 | 30 | 4 | 1.4 ± 0.1 | 2.0 | 5 | ||||
| D206E | 1.5 ± 0.1 | 10 | 8 | 0.74 ± 0.02 | 1.1 | 6 | ||||
| D206F | 7 ± 4 | 45 | 3 | 2.0 ± 0.1 | 2.9 | 3 | ||||
| D206K | 13 ± 15 | 80 | 3 | 4.7 ± 0.2 | 6.7 | 3 | ||||
| Y231 | ||||||||||
| Y231A | 53 ± 10 | 350 | 3 | 1.6 ± 0.4 | 2.3 | 3 | ||||
| Y231C | 26 ± 8 | 170 | 6 | 1.6 ± 0.6 | 2.3 | 6 | ||||
| V262 | ||||||||||
| V262A | 0.41 ± 0.04 | 2.6 | 3 | 0.9 ± 0.1 | 1.3 | 3 | ||||
| V262C | 0.35 ± 0.03 | 2 | 3 | 0.7 ± 0.1 | 1.0 | 4 | ||||
| V262D | 8 ± 2 | 50 | 4 | 0.9 ± 0.1 | 1.3 | 3 | ||||
| V262F | 8 ± 2 | 50 | 3 | 1.6 ± 0.3 | 2.3 | 4 | ||||
| V262G | 0.87 ± 0.04 | 5.6 | 5 | 1.2 ± 0.3 | 1.7 | 3 | ||||
| V262I | 0.09 ± 0.001 | 0.55 | 5 | 0.9 ± 0.1 | 1.3 | 3 | ||||
| Y282 | ||||||||||
| Y282C | 0.8 ± 0.2 | 5 | 9 | 0.7 ± 0.1 | 1 | 7 | ||||
| Y282S | 0.7 ± 0.1 | 4.5 | 3 | 1.6 ± 0.6 | 2.3 | 3 | ||||
| Y282W | 0.24 ± 0.02 | 1.5 | 4 | 0.9 ± 0.1 | 1.3 | 3 | ||||
| NTD deletion | ||||||||||
| NR2B-ΔNTD | 145 ± 3 | 900 | 3 | 11 ± 1 | 16 | 3 | ||||