TABLE 1

Binding characteristics for the α_2A-adrenergic receptor constructs

For saturation binding, cell membranes were incubated for 90 min with 1 to 110 nM [³H]RX821002 in binding buffer (5 mM Tris-HCl and 2 mM EDTA, pH 7.4). Average data from two independent experiments are presented for saturation experiments. Competition binding was then performed with 4 nM [³H]RX821002 and different concentrations of each agonist. Three to four competition experiments were performed for each compound. Nonspecific binding was determined in the presence of 1 μM atipamezole. Saturation and competition binding studies were analyzed with the program Origin to calculate K_D and K_i values and the 95% confidence intervals.