Oligonucleotide and Orientation | Sequence (5′ to 3′) | Position | |
---|---|---|---|
Primers used for the combined bisulfite restriction analysis and the bisulfite PCR | |||
Oatp1b2 | |||
−594-F | GGTTATTTGATTTTTGATAAAGGAGT | −594 to −569 | |
−347-R | TACCCACTACTTAAAAACATTCAATA | −347 to −372 | |
−40-F | GTAGAGAGGTTTTAAAGTGGGAT | −40 to −18 | |
+208-R | ACCTAAACCACATCTATCTCCTA | +208 to +186 | |
Ntcp | |||
−542-F | GTAAGGTTTTAGAGGAGGAT | −542 to −523 | |
−1-R | CCTTCCTTACCCAATACTAA | −1 to −20 | |
−46-F | GAAAGAGGATTGAAGAAAAT | −46 to −20 | |
+522-R | CAATACCAAACTTAATCCAA | +522 to +503 | |
+179-F | GAGTTTAGTAAGATTAAGGT | +179 to +198 | |
+671-R | TCTCCTTTACAATAATCCTA | +671 to +652 | |
Bsep | |||
−404-F | GGTTTTAGATTATTGAGGGTTTGT | −404 to −381 | |
−90-R | CCTAAAATCCAAATCTAACACAATTCAA | −90 to −117 | |
−110-F | GTGTTAGATTTGGATTTTAGGTT | −110 to −88 | |
+340-R | ACACACACACACACATTTTTAAA | +340 to +318 | |
+416-R | CCAAATTTAAAAAACATCTCTCTA | +416 to +393 | |
Abcg5 | |||
−315-F | TAGTTATGATTAGTGTTGTTTGTGT | −315 to −291 | |
+125-R | CAAAAAACTCAAAAACCCTCTATTA | +125 to +100 | |
+1035-F | GGTATTTGAATATAATTTGGTGAT | +1035 to +1058 | |
+1212-R | TAAACATACCCAAAACTCTA | +1212 to +1193 | |
Pept2 | |||
−669-F | GTTGAATGTTATGATGTTGAAAATT | −669 to −645 | |
−144-R | TACAACATCTTAACTAAACATACAA | −144 to −168 | |
−171-F | GAGTTGTATGTTTAGTTAAGATGTTGT | −171 to −145 | |
+155-R | AACTAAAACTACTACCAACCTACAATA | +155 to +129 | |
Primers used for the RT-PCR | |||
Oatp1b2 | |||
Forward | GGAGGGTGCAGAGCTAGATG | +471 to +490 | |
Reverse | ATTCCTCGAGATCCCCTTGT | +671 to +652 | |
Ntcp | |||
Forward | GTTTAAATCCCAGGGGGAAA | +571 to +590 | |
Reverse | CGTTTGGTTCCAAGTCACCT | +807 to +788 | |
Bsep | |||
Forward | AGCTTCCGTTGTTCTCAGGA | +496 to +515 | |
Reverse | GGTGGAGAGGAGGGAAGAAC | +684 to +665 | |
Abcg5 | |||
Forward | TGGTGGCCAACTTGTCATTA | +622 to +641 | |
Reverse | CAAGGGCAAAGGCAGAGTAG | +774 to +755 | |
Abcg8 | |||
Forward | TTGGCATTGTTTGGTGAGAA | +524 to +543 | |
Reverse | AAGGTAGCGAGATGCCTCAA | +696 to +677 | |
Pept2 | |||
Forward | TGAGTCCAAGGAAACGCTCT | +224 to +243 | |
Reverse | CCAACAGCCAACGAGAAACT | +384 to +365 | |
β-actin | |||
Forward | CAGCCAACTTTACGCCTAGC | +208 to +227 | |
Reverse | GGTTTGGACAAAGACCCAGA | +453 to +434 | |
Primers used for the real-time PCR in ChIP assay | |||
Oatp1b2 | |||
Forward | TTGTGTAGGACAGTGACCTGAAG | −94 to −72 | |
Reverse | GGCATGTCTAACCTGCACCT | +12 to −8 | |
Ntcp | |||
Forward | ATCCGGGGAGATGAGGTTAT | −154 to −135 | |
Reverse | CCACCTCACTCTCTGCTCCT | +22 to +3 | |
Bsep | |||
Forward | TTTGGGTTCCTGCTTTGAGT | −162 to −143 | |
Reverse | GGTTGTACACCCCTTTCCAA | +12 to −8 | |
Abcg5 | |||
Forward | TCCTCTCTGGCAAACACTCC | −192 to −173 | |
Reverse | CAGGGCAGCTTTAACTGAGG | −1 to −20 | |
Pept2 | |||
Forward | TCTGAAATCAGCCTTCTGTGTT | −278 to −257 | |
Reverse | CACGTCACAAGTCACGAGATG | −38 to −58 | |
β-actin | |||
Forward | AAATGCTGCACTGTGCGGCG | −139 to −120 | |
Reverse | AGGCAACTTTCGGAACGGCG | −63 to −82 |
F, forward; R, reverse.