TABLE 1
Overexpression of Gαq and Gα16 proteins in hCRF1 - and hCRF2(a)-HEK cells
Medium effective concentration (EC50) and maximal concentration (Emax) were determined in the Ca2+ mobilization in hCRF1 - and hCRF2(a) cells transfected with Gαq and Gα16. The results are expressed as the percentage of the Ca2+ response in cells transfected with either Gαq or Gα16 compared with cells transfected with empty vector pcDNA3 (mock). The data are means ± S.E.M. of three to six independent stimulation experiments performed in triplicate. The values are expressed as the percentage of response to the maximal response measured on the mock-transfected cells.