CHO-V1b | HEK-293-V1b | COS-7-V1b | |
---|---|---|---|
Binding [3H]AVP | |||
Kd (AVP) (nM) | 5.10 ± 0.68 | 3.71 ± 0.70 | N.D. |
Bmax (AVP) (pmoles/mg protein) | 3.58 ± 0.09 | 16.7 ± 2.3 | N.D. |
V1b receptors/cell (×10−3) | 448 ± 27 | 796 ± 171 | N.D. |
EC50 (nM) | |||
IP1 accumulation | 6.98 ± 0.77 | 7.28 ± 0.75 | 3.84 ± 0.54 |
cAMP accumulation | 22.2 ± 1.6 | 32.7 ± 2.4 | 18.6 ± 3.9 |
Hill coefficient | |||
IP1 accumulation | 0.78 ± 0.07 | 0.80 ± 0.06 | 1.11 ± 0.15 |
cAMP accumulation | 1.07 ± 0.07 | 1.42 ± 0.13 | 0.78 ± 0.12 |
Maximal value (pmol/well) | |||
IP1 accumulation | 29.2 ± 0.6 | 23.6 ± 0.5 | 77.8 ± 1.6 |
cAMP accumulation | 1.56 ± 0.03 | 0.36 ± 0.02 | 6.41 ± 0.12 |
N.D., not determined because of the expression of endogenous V2 receptor.