Effects of gene deletion on high- and low-sensitivity components of ACh-evoked [3H]GABA release
Rmax and EC50 values were calculated from concentration-response curves as described under Materials and Methods. Each value represents the mean ± S.E.M. as compiled from four to eight individual concentration-effect curves for each genotype. The two components of the concentration-response curve are designated as high-ACh sensitivity (HS) and low-ACh sensitivity (LS).
Genotype | Rmax (HS) | logEC50 (HS) | Rmax (LS) | logEC50 (LS) |
|---|---|---|---|---|
| μM | μM | |||
| Cortex | ||||
| α4(+/+) | 100.31 ± 11.80 | −0.17 ± 0.098 | 39.78 ± 25.21 | 1.40 ± 0.480 |
| α4(−/−) | ||||
| β2(+/+) | 80.58 ± 29.56 | −0.44 ± 0.381 | 15.06 ± 15.05 | 1.35 ± 0.66 |
| β2(−/−) | ||||
| α5(+/+) | 84.45 ± 13.16 | −0.32 ± 0.171 | 50.10 ± 10.36 | 0.93 ± 0.45 |
| α5(−/−) | 40.15 ± 6.48 | −0.66 ± 0.14 | 56.37 ± 17.85 | 1.31 ± 0.52 |
| Hippocampus | ||||
| α4(+/+) | 81.28 ± 16.13 | −0.34 ± 0.199 | 53.03 ± 16.72 | 2.14 ± 0.28 |
| α4(−/−) | ||||
| β2(+/+) | 77.98 ± 6.46 | −0.44 ± 0.13 | 105.28 ± 39.65 | 2.20 ± 0.23 |
| β2(−/−) | ||||
| α5(+/+) | 69.40 ± 10.92 | −3.42 ± 2.70 | 61.24 ± 7.11 | 1.27 ± 0.53 |
| α5(−/−) | 51.30 ± 14.31 | −1.98 ± 1.43 | 51.23 ± 9.84 | 1.20 ± 0.20 |
| Striatum | ||||
| α4(+/+) | 42.83 ± 4.31 | −1.12 ± 0.15 | 64.98 ± 24.01 | 1.00 ± 0.55 |
| α4(−/−) | ||||
| β2(+/+) | 48.10 ± 8.03 | −1.67 ± 0.62 | 60.35 ± 9.02 | 0.60 ± 0.21 |
| β2(−/−) | ||||
| α5(+/+) | 51.79 ± 6.96 | −0.73 ± 0.18 | 63.17 ± 12.12 | 0.998 ± 0.12 |
| α5(−/−) | 51.18 ± 9.37 | −0.46 ± 0.18 | 41.61 ± 13.68 | 0.82 ± 0.47 |
| Thalamus | ||||
| α4(+/+) | 54.30 ± 10.31 | −2.59 ± 2.10 | 62.98 ± 25.91 | 1.16 ± 0.62 |
| α4(−/−) | ||||
| β2(+/+) | 40.58 ± 8.24 | −2.37 ± 1.29 | 75.72 ± 17.67 | 0.94 ± 0.44 |
| β2(−/−) | ||||
| α5(+/+) | 41.54 ± 5.23 | −0.33 ± 0.20 | 82.71 ± 22.09 | 1.46 ± 0.21 |
| α5(−/−) | 47.17 ± 12.00 | −2.75 ± 2.13 | 47.10 ± 17.77 | 1.00 ± 0.40 |