TABLE 2

Agonist binding affinities of the dopamine D2 receptor expressed alone or coexpressed with the dopamine D5 receptor

Competition binding experiments using [3H]raclopride were performed in membranes from cells stably transfected with the D2 receptor alone or cotransfected with the D5 receptor. The data represent the means ± S.E.M of six experiments, each performed in duplicate.



D2R

D2R + D5R

Fraction
KH
KL
Fraction
KH
KL
nM nM nM nM
Dopamine 0.291 ± 0.084 256.6 ± 105.3 4836 ± 1612 0.271 ± 0.073 144.0 ± 84.4 4030 ± 1113
Quinpirole
0.293 ± 0.047
13.8 ± 8.7
1196 ± 366
0.253 ± 0.034
25.3 ± 3.4
875.5 ± 270.1
  • KH, high-affinity constant; KL, low-affinity constant