Effect of GRKs and GRK mutant on pERK1/2 stimulation: quantification of immunoblots
Single experiments are shown in Figs. 4 and 5. Data are expressed as the ratio of the respective control values (controls = 1). Values are means ± S.E.M. from three to five determinations.
mGlu2R | mGlu2R + GRK2 | mGlu2R + GRK5 | mGlu2R + K220R | mGlu3R | mGlu3R + GRK2 | mGlu3R + GRK5 | mGlu3R + K220R | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | pERK1/ERK1 | pERK2/ERK2 | ||||||||
| 0′ | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||||||
| 5′ | 2.5 ± 0.3 | 4.1 ± 0.5 | 1.4 ± 0.2* | 1.6 ± 0.1* | 2.4 ± 0.0 | 4.1 ± 0.3 | 1.6 ± 0.4 | 4.2 ± 0.0 | 2.7 ± 0.1 | 3.1 ± 0.4 | 1.5 ± 0.1* | 1.6 ± 0.1* | 2.5 ± 0.1 | 2.8 ± 0.1 | 2.5 ± 0.1 | 3.1 ± 0.2 | ||||||||
| 15′ | 1.5 ± 0.4 | 1.6 ± 0.3 | 1.0 ± 0.0 | 0.8 ± 0.1 | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.9 ± 0.3 | 2.1 ± 0.5 | 1.1 ± 0.1 | 0.9 ± 0.1 | 1.5 ± 0.3 | 1.6 ± 0.4 | 1.5 ± 0.4 | 1.7 ± 0.3 | ||||||||
| 30′ | 0.9 ± 0.1 | 1.3 ± 0.2 | 0.1 ± 0.1 | 0.8 ± 0.1 | 1.1 ± 0.1 | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.1 ± 0.1 | 1.4 ± 0.2 | 1.5 ± 0.1 | 1.0 ± 0.0 | 0.8 ± 0.1 | 1.0 ± 0.1 | 1.0 ± 0.1 | 0.9 ± 0.2 | 0.9 ± 0.1 | ||||||||
↵* p < 0.05 (one-way ANOVA plus Fisher's t test) vs control values.