TABLE 1

Kinetics of recovery of ACh responses after inhibition by azietomidate

nAChRs in detached patches were exposed for 2 s to 300 μM ACh ± 10 μM azietomidate, and then returned to buffer, with ACh sensitivity monitored at intervals by moving the patch for 50 ms to a test solution of 300 μM ACh + azietomidate (Fig. 3). I₀ is the peak initial response to 300 μM ACh ± 10 μM azietomidate; I_f is the current after 1.95 s. The observed recovery was fit to a single exponential: I = I_rec - a_s exp^−bt, where I_rec is the current after full recovery, a_s is the amplitude of the observed slow recovery on the time scale of seconds, and b is the rate constant. The amplitude of the rapid phase of recovery, a_f, is equal to I_rec − (a_s + I_f). See Figure 3A for a definition of the parameters.