Primer sets and probe for RT-PCR and real-time PCR
Each position is from the sequence in the GenBank database.
Gene Names (Accession No.) | Position | Sequences (5′ to 3′) |
|---|---|---|
| For RT-PCR | ||
| Mouse Matel (NM_026183) | ||
| Forward primer | 18 to 35 | ACGGAGGCCACATGGAAC |
| Reverse primer | 2036 to 2017 | TCCACTCCAGAGCATCTCCT |
| Mouse Gapdh (NM_008084) | ||
| Forward primer | 70 to 90 | CAGTGCATCTTCTTGTGCAGT |
| Reverse primer | 1238 to 1218 | TGTGAGGGAGATGCTCAATGT |
| For real-time PCR | ||
| Mouse Mate2 (NM_001033542) | ||
| Forward primer | 1521 to 1540 | TTCGAGCTGGGCTAAAAAGC |
| Reverse primer | 1589 to 1568 | CTCTTTCCAAGATGGGCAGATC |
| TaqMan probe | 1542 to 1566 | CCAAGGAGTTGATACCCACGCCAGC |