pKi | Bmax | |||||
---|---|---|---|---|---|---|
High-Affinity Site | Low-Affinity Site | High-Affinity Site | Low-Affinity Site | |||
pmol/mg | ||||||
WT | 8.62 ± 0.15 | 6.52 ± 0.19 | 7.2 ± 1.5 | 187 ± 3 | ||
WT (GppNHp) | 8.03 ± 0.22* | 6.48 ± 0.22 | 9.1 ± 2.3 | 127 ± 36 | ||
G243A/I247A | 8.14 ± 0.08 | 6.55 ± 0.22 | 9.7 ± 1.5 | 138 ± 36 | ||
G243A/I247A (GppNHp) | 7.87 ± 0.07 | 6.31 ± 0.17 | 8.5 ± 0.8 | 152 ± 42 |
↵* Significantly different from the same receptor construct studied in the absence of GppNHp, P < 0.05.