Deactivation kinetics of GluR6 mutants
τDec-1 ms and τDec-SS are the deactivation rate from peak and from steady state, respectively, determined as described under Materials and Methods. Values are presented as mean ± S.E.M. for n determinations. Boldface type indicates significant differences comparing τDec-1 ms or τDec-SS values with those of GluR6 wild type. “Quad” is the GluR6 K525E/K696R/I780L/Q784K mutant characterized in Zhang et al. (2006).
| Mutant | Glu | KA | ||||||
|---|---|---|---|---|---|---|---|---|
| τDec-1 ms | n | τDec-SS | n | τDec-1 ms | n | τDec-SS | n | |
| Wild type | 3.9 ± 0.2 | 17 | 10.8 ± 2.4 | 6 | 4.4 ± 0.2 | 17 | 127 ± 32 | 5 |
| “Quad” | 37 ± 2.2 *** | 4 | 93 ± 16 *** | 12 | N.D. | 1050 ± 170 | 12 | |
| K531E | 2.5 ± 0.3 | 10 | N.D. | 14.2 ± 1.0 *** | 10 | N.D. | ||
| D776K | 3.9 ± 0.3 | 18 | 5.7 ± 0.4 * | 25 | 47 ± 2.3 *** | 18 | 57 ± 3.9 | 21 |