τh of inactivation at −10 mV of wild-type and mutants in the absence and presence of internal QX314
Values are presented as mean ± S.E.M.
| Control | 250 μM QX314 | |||
|---|---|---|---|---|
| n | τh | n | τh | |
| ms | ms | |||
| WT | 12 | 0.59 ± 0.04 | 6 | 0.66 ± 0.05 |
| R395C | 5 | 0.60 ± 0.06 | 5 | 0.63 ± 0.06 |
| L396C | 5 | 0.81* ± 0.10 | 5 | 0.83 ± 0.09 |
| M397C | 5 | 0.67 ± 0.10 | 5 | 0.64 ± 0.08 |
| T398C | 6 | 0.62 ± 0.09 | 6 | 0.71 ± 0.08 |
| Q399C | 8 | 0.55 ± 0.06 | 8 | 0.62 ± 0.07 |
| C753A | 5 | 0.59 ± 0.06 | 5 | 0.64 ± 0.08 |
| T1235C | 5 | 0.68 ± 0.05 | 4 | 0.66 ± 0.06 |
| F1236C | 7 | 0.69 ± 0.08 | 7 | 0.67 ± 0.07 |
| S1528C | 7 | 0.62 ± 0.04 | 7 | 0.64 ± 0.06 |
| A1529C | 5 | 0.69 ± 0.06 | 5 | 0.65 ± 0.09 |
* P < 0.05 verus WT in control.