On and off rates and dissociation constants of selective mutants
Values are presented as mean ± S.E.M.
| τon | τoff | koff | kon | kd | n | |
|---|---|---|---|---|---|---|
| s | min | ms −1 | μM−1·s−1 | μM | ||
| WT | 7.66 ± 0.72 | 9.44 ± 1.21 | 1.77 ± 0.14 | 51.50 ± 4.83 | 3.43 ± 0.40 | 5 |
| Q399C | 11.84 ± 0.85* | 4.55 ± 0.47* | 3.67 ± 0.4*2 | 32.31 ± 1.24* | 11.35 ± 0.69* | 5–10 |
| F1236C | 8.82 ± 0.91 | 1.41 ± 0.19* | 11.78 ± 0.90* | 40.61 ± 3.66 | 29.02 ± 3.34* | 6 |
* P < 0.05 versus WT.