TABLE 1

Summary of the effects of DAMGO, EGF, or DAMGO + EGF on DAMGO-stimulated GTPγS binding on wild-type or mutant Y166F MOR

Pharmacological data for DAMGO-stimulated GTPγS binding was collected on membranes taken from HEK cells stably transfected with either wild-type MOR (MOR) or the MOR mutant Y166F (Y166F). GTPγS binding was measured and analyzed as described under Materials and Methods. Results represent the means ± S.E.M. of four to eight independent experiments performed in triplicate. Data were analyzed by one-way ANOVA followed by Tukey's post hoc test. EC₅₀ values were determined from nonlinear regression analysis (Prism version 4.0).