Sequences and modifications of the helix 9 peptides
ET residues are underlined. In peptides 6, 7, and 8, Nle was substituted for Met165 to avoid sulfur oxidation.
| Peptide | Sequence | Modification |
|---|---|---|
| 1 | EFDRRWRQWRELWLR | Modified sequence except for ET residues, designed by PB |
| 1AA | Ac-EFDRRWRQWRELWLR-NH2 | (AA) Acetylated and amidated peptide 1 |
| 2 | DFEERRRQWLILYR | Modified sequence except for ET residues, designed by PB |
| 2AA | Ac-DFEERRRQWLILYR-NH2 | (AA) Acetylated and amidated peptide 2 |
| 3 | EEYFKRRWEQWYKLY | Modified sequence except for ET residues, designed by PB |
| 4 | YFNRFLQWKWLE | Unmodified GRK6 α9 (166–177) |
| 5 | Ac-FFDRFLQWKWLE-NH2 | Acetylated and amidated GRK5 α9 (166–177) |
| 6 | Ac-SNleFFDRFLQWKWLER-NH2 | Acetylated and amidated GRK5 α9 (164-M165Nle-178) |
| 7 | Ac-SNleFFDRELQWKWLER-NH2 | Glu170 side chain–Lys174 side chain bridge |
| 8 | Ac-SNleFEDRFKQWKWLER-NH2 | Glu167 side chain–Lys171 side chain bridge |
PB, peptide builder.