TABLE 2

Long-term fluoxetine modulates basal AC activity and 5-HT1A receptor mediated inhibition of AC in the hippocampus

Imax and pEC50 values correspond to the estimated maximal effect and half-maximal effect of the 5-HT1A receptor agonist 8-OH-DPAT in AC assays. Values are means ± S.E.M. of two independent experiments performed in brain samples from eight to nine animals for each condition.

VehicleFluoxetineFluoxetine + WAY100635WAY100635
Basal AC activity (pmol cAMP/min/mg protein)11.5 ± 0.616.8 ± 1.2*12.4 ± 1.3#11.3 ± 1.8#
Forskolin-stimulated AC activity (pmol cAMP/min/mg protein)92 ± 18113 ± 15101 ± 696 ± 10
WIN55,212–2 inhibition of forskolin-stimulated AC activity
    Imax (%)27.1 ± 1.915.6 ± 1.9**21.8 ± 3.122.1 ± 2.4
    pEC507.8 ± 0.37.4 ± 0.47.5 ± 0.38.1 ± 0.4

  • ↵* p < 0.05 versus vehicle.

  • ↵# P < 0.05 versus fluoxetine (Newman-Keuls post-ANOVA).

  • ↵** p < 0.01 versus vehicle.