Long-term treatment with fluoxetine and/or WAY100635 does not modulate PKA_c, PKAr1α, CREB, pCREB, ERK1/2, and pERK1/2 expression in total cell lysate fractions of rat cerebellum
Values are means ± S.E.M. corresponding to densitometric analysis of the different proteins in total cell lysate fractions of cerebellum from rats treated with fluoxetine, fluoxetine + WAY100635, or WAY100635 alone, expressed as percentage of the same proteins in vehicle-treated animals. Two independent experiments were performed in brain samples from five animals for each condition.
| Vehicle | Fluoxetine | Fluoxetine + WAY100635 | WAY100635 | |
|---|---|---|---|---|
| PKA_c | 100 ± 7 | 114 ± 13 | 92 ± 11 | 99 ± 7 |
| PKA_r1α | 100 ± 6 | 91 ± 9 | 96 ± 7 | 98 ± 5 |
| CREB | 100 ± 11 | 100 ± 20 | 97 ± 25 | 118 ± 18 |
| pCREB | 100 ± 5 | 102 ± 12 | 104 ± 10 | 113 ± 10 |
| ERK1 | 100 ± 16 | 105 ± 7 | 119 ± 7 | 115 ± 11 |
| ERK2 | 100 ± 12 | 90 ± 2 | 86 ± 5 | 94 ± 4 |
| pERK1 | 100 ± 4 | 93 ± 8 | 102 ± 3 | 102 ± 6 |
| pERK2 | 100 ± 4 | 93 ± 10 | 115 ± 12 | 114 ± 10 |