TABLE 2

Negative logarithm of antagonist equilibrium dissociation constants (pKB) as estimated from Gaddum analysis of functional inhibition of 1 to 100 nM NECA in the absence and presence of Brilliant Black BN at the adenosine A1 and A3 receptors

Data represent the mean ± S.E.M. of three to nine experiments conducted in duplicate.

Brilliant Black BN
0 μM500 μM
A1-CHO
    DPCPX8.6 ± 0.18.4 ± 0.1
    XAC-X-BY6306.9 ± 0.15.3 ± 0.2*
    XACa7.1 ± 0.15.6 ± 0.1*
A3-CHO
    MRS12209.7 ± 0.19.1 ± 0.1*
    XAC-X-BY6307.7 ± 0.25.9 ± 0.1*
    XACb7.5 ± 0.16.0 ± 0.1*

  • ↵a pKB values represent the average calculated from the inhibition of 1 nM and 10 nM NECA. There was no significant difference (P > 0.05; unpaired t test) between corresponding pKB values determined in the presence of 1 nM and 10 nM NECA.

  • ↵b pKB values represent the average calculated from the inhibition of 10 nM and 100 nM NECA. There was no significant difference (P > 0.05; unpaired t test) between corresponding pKB values determined in the presence of 10 nM and 100 nM NECA.

  • ↵* Significantly different (P < 0.05; unpaired t test) from the corresponding value in the absence of Brilliant Black BN.