TABLE 4 Thermodynamics of IP3 and adenophostin A binding to the NT and IBC
From the effects of temperature on IP3 and adenophostin A binding to the IBC and NT in CLM (Table 3, Fig. 4, and Supplemental Fig. 6), ΔG was determined (ΔG = R · T lnKD) and thereby ΔH and ΔS (eq. 3, assuming ΔC = 0). A similar analysis in which ΔC was not fixed to 0 provided indistinguishable results (Supplemental Table 1). −TΔS is also shown for 296 K. Results are means ± S.E.M., from three separate van't Hoff plots.