TABLE 4

Thermodynamics of IP₃ and adenophostin A binding to the NT and IBC

From the effects of temperature on IP₃ and adenophostin A binding to the IBC and NT in CLM (Table 3, Fig. 4, and Supplemental Fig. 6), ΔG was determined (ΔG = R · T lnK_D) and thereby ΔH and ΔS (eq. 3, assuming ΔC = 0). A similar analysis in which ΔC was not fixed to 0 provided indistinguishable results (Supplemental Table 1). −TΔS is also shown for 296 K. Results are means ± S.E.M., from three separate van't Hoff plots.

	ΔG^a	ΔH	ΔS	−TΔS^a
	kJ/mol		J/mol·K	kJ/mol
IP₃
NT	−37.1 ± 0.1	−37.0 ± 1.1	0.9 ± 3.7	−0.3 ± 1.1
IBC	−43.2 ± 0.1	−36.8 ± 0.3	21.3 ± 1.5	−6.3 ± 0.4
Adenophostin A
NT	−43.5 ± 0.02	−32.1 ± 1.1	35.6 ± 3.7	−10.5 ± 1.1
IBC	−49.4 ± 0.2	−21.9 ± 1.0	93 ± 3.6	−27.5 ± 1.1

↵a 296 K.