Before UV | After 8-h UV | |||
---|---|---|---|---|
Imax | N | Imax | N | |
μA | μA | |||
αβ | −9 ± 1 | 8 | −5 ± 1 | 7 |
αMet113Met | −1.9 ± 0.5 | 9 | −2.5 ± 0.7 | 5 |
αMet113Npg | −1.5 ± 0.2 | 23 | −0.17 ± 0.03* | 24 |
↵* Statistically significant (P < 0.01) difference between the Imaxvalue before and after 8 h of UV exposure. Similar comparisons between the wild-type (αβ) and αM113M data sets showed no significant differences. Higher expression of the wild-type receptor caused greater scatter in the Imax values. Although the Imax value after exposure to UV light is ∼55% of the before UV light value, this range of expression levels for the wild-type receptor is normal for a given data set. Suppression experiments generally give less variability in expression size.