Operational model parameters for the interaction between CCh and brucine at the M3K7.32E mAChR
Data are presented as the mean ± S.E.M. of three to six separate experiments performed in duplicate.
| Ca2+ Mobilization | Membrane Ruffling | ERK1/2 Phosphorylation | ERK1/2 Phosphorylation + PTX | |
|---|---|---|---|---|
| Log KB | −5.17 ± 0.15 | −4.61 ± 0.13 | −5.21 ± 0.28 | −5.30 ± 0.19 |
| Log τB | 0.41 ± 0.03** (τB = 2.6) | −1.40 ± 0.48 (τB = 0.04) | −0.66 ± 0.16 (τB = 0.2) | −0.43 ± 0.16 (τB = 0.4) |
| Log αβ | 0.70 ± 0.17 (αβ = 5.0) | 0.88 ± 0.10 (αβ = 7.6) | 0.84 ± 0.06 (αβ = 6.9) | 0.84 ± 0.07 (αβ = 6.9) |
Log KB, logarithm of the dissociation constant of the allosteric modulator; Log τB, logarithm of the operational efficacy of the allosteric modulator; Log αβ, logarithm of the cooperativity of the allosteric modulator on the potency of the orthosteric agonist.
↵** P < 0.01 determined by one-way ANOVA with Newman-Keuls multiple comparisons post test across Log τB values (statistically different to the Log τB value derived for membrane ruffling).