CCh potency (pEC50) and curve-translocation (Log δ) values for the interaction between CCh and brucine at the M3K7.32E mAChR after transfection with siRNA
Data are presented as the mean ± S.E.M. of four to five separate experiments performed in duplicate.
| Transfection Control | Gαq siRNA | Gα12 siRNA | |
|---|---|---|---|
| Ca2+ mobilization | |||
| pEC50 | 7.16 ± 0.20 | 7.44 ± 0.12 | 6.99 ± 0.16 |
| Log δ | 1.14 ± 0.23 | 0.41 ± 0.12* | 1.01 ± 0.19 |
| ERK1/2 phosphorylation | |||
| pEC50 | 6.33 ± 0.11 | 6.28 ± 0.12 | 6.64 ± 0.10 |
| Log δ | 0.82 ± 0.13 | 0.62 ± 0.14 | 0.16 ± 0.13* |
pEC50, negative logarithm of concentration agonist that generates 50% of the maximal agonist response; Log δ, logarithm of the ratio of the EC50 values of the allosteric modulator-treated and control CCh curves.
↵* P < 0.05 determined by one-way ANOVA with Newman-Keuls multiple comparisons post test between each transfection group in a single assay type.