GTPase stimulation by RGS4 mutants
| Rate ± S.E.M. (n = 3) | |||
|---|---|---|---|
| Without Inhibition | +100 μM CCG-4986 | Inhibition | |
| ×10−2/s | % | ||
| Gαo alone | 0.11 ± 0.02 | ||
| RGS4 | 2.4 ± 1.4 | 0.75 ± 0.48 | 69 |
| C132A | 2.2 ± 0.26 | 0.22 ± 0.01 | 90 |
| C148A | 1.4 ± 0.6 | 0.19 ± 0.05 | 86 |
| A132C | 1.2 ± 0.15 | 0.34 ± 0.1 | 72 |
| A148C | 4.5 ± 0.48 | 1.1 ± 0.39 | 76 |
| Cysless | 0.42 ± 0.06 | 0.36 ± 0.03 | 14 |