TABLE 2

Global analysis of ABA-X-BY630 (30 nM) kinetic and equilibrium binding parameters in the absence and presence of PD81,723 and VUF5455 at the A1AR and A3AR

Data are expressed as the mean ± S.E.M. of the stated number of replicates (n) where each n represents the average of 10 cells within a single experiment.

ABA-X-BY630 (30 nM)konkoffpKD(APPARENT)BMAX (Fluorescence Intensity)n
× 107 M−1min−1min−1M−1
CHO-A1
    Control2.62 ± 0.341.95 ± 0.097.13 ± 0.06150 ± 1612
    + 1 μM PD81,7232.88 ± 0.391.72 ± 0.077.23 ± 0.0610
    + 10 μM PD81,7234.17 ± 0.65*0.80 ± 0.03*7.72 ± 0.07*6
    + 0.1 μM VUF54552.82 ± 0.401.60 ± 0.107.25 ± 0.063
    + 1 μM VUF54551.04 ± 0.161.48 ± 0.16*6.85 ± 0.07*5
CHO-A3
    Control8.60 ± 0.620.78 ± 0.028.05 ± 0.04133 ± 414
    + 1 μM PD81,7236.78 ± 0.45*1.13 ± 0.04*7.78 ± 0.03*9
    + 10 μM PD81,7234.79 ± 0.38*2.46 ± 0.18*7.23 ± 0.03*6
    + 0.1 μM VUF54554.23 ± 0.35*2.73 ± 0.22*7.12 ± 0.03*7

  • ↵* Statistically different (P < 0.05) compared with control value, as determined by one-way ANOVA followed by a Dunnett's multiple comparisons test.