TABLE 2

RGS specificity of CCG-63802 and CCG-63808 determined by multiplex FCPIA analysis (n ≥ 3)

All data were calculated from at least three independent experiments using nonlinear least-squares regression with the bottom of the curves constrained to 0% binding.

RGS Protein	CCG-63802		CCG-63808
RGS Protein	IC₅₀	Hill Slope	IC₅₀	Hill Slope
	μM		μM
RGS4	9	−0.9	10	−1.4
RGS4c	>400	−0.4	>400	−0.8
RGS8	112	−0.6	74	−1.1
RGS16	42	−1.4	21	−2.1
RGS19	20	−0.6	46	−0.8
RGS7	N.I.	N.I.	N.I.	N.I.

N.I., no inhibition observed at highest concentration tested (100 μM).