TABLE 2 Comparison of the inhibition induced by MRT compounds on ShhN-induced Gli-dependent luciferase activity in Shh-light2 cells and SAG-induced differentiation of C3H10T1/2 cells
The structure of MRT compounds identified in the first and second virtual library screening procedure are shown. The values correspond to percentage inhibition of the luciferase activity induced by ShhN (5 nM) in the presence of 3 μM concentrations of each compound analyzed in the Shh-light2 assay. The values correspond to percentage inhibition of AP activity induced by SAG (0.1 μM) in the presence of 10 μM of each compound analyzed in C3H10T1/2 cell differentiation assay. Data are the means ± S.E.M. of at least three independent experiments.