Transfection efficiency and fluorescence intensity of GFP-fused chimeras
MDCK cells were transfected with the GFP-fused chimeras; after 48 h, cells were resuspended and analyzed by flow cytometry for transfection efficiency and construction expression rate. Fluorescence intensity is expressed in arbitrary units. Data are expressed as the mean ± S.D. of three experiments carried out on different days on different batches of cells.
| Construction | Transfection | S.D. | Fluorescence Intensity | S.D. |
|---|---|---|---|---|
| % | ||||
| hCNT3 | 39.6 | 0.5 | 60.3 | 4.1 |
| hCNT3Δ10 | 36.4 | 0.3 | 61.1 | 2.9 |
| hCNT3Δ25 | 36.8 | 0.5 | 64.0 | 0.3 |
| hCNT3Δ69 | 38.5 | 0.3 | 11.0 | 4.4 |
| hCNT317VGFQ20MTFR | 38.2 | 0.3 | 62.2 | 4.1 |