TABLE 1

Primers and probes

Probes are FAM-5′→3′-TAMRA; primers are 5′→3′.

Species & Gene	GenBank Accession No.	Probe/Primers	Sequence
Rat
G6Pase	NM_013098	Probe	`TGGGTGGCAGTGGTTGGAGACT`
		Forward	`TTCAAGAGACTGTGGGCATCAAT`
		Reverse	`ATCCACTTAAAGACGAGGTTG`
PEPCK	NM_198780	Probe	`CTATGTGGTGGCCAGCATGCG`
		Forward	`AAGATTGGTATTGAGCTGACAGAC`
		Reverse	`AGAGGTCCCCATCCGTGT`
Foxo1	XM_342244	Probe	`ATGGCCGTGCCCTGCCCC`
		Forward	`CATGCACAGCAAACTTCTTCAGT`
		Reverse	`TGTGTGAGGCATGGTGTTCA`
Mouse
G6Pase	NM_008061	Probe	`TGGGTGGCAGTGGTCGGAGACT`
		Forward	`TTAAAGAGACTGTGGGCATCAAT`
		Reverse	`ATCCACTTGAAGACGAGGTTG`
PEPCK	NM_011044	Probe	`CTATGTGGTGGCCAGCATGCG`
		Forward	`GGTATTGAACTGACAGACTCGC`
		Reverse	`CACAGATATGCCCATCCGA`
Foxo1	NM_019739	Probe	`CCCTTTGCCCCAGATGCCTATG`
		Forward	`AAGTACAGATACGGCCAATCC`
		Reverse	`CGTAACTTGATTTGCTGTCCTG`
apoCIII	NM_023114	Probe	`CTGACAAGTTCACCGGCTTCTGGGA`
		Forward	`TGAAAGGCTACTGGAGCAAGTTT`
		Reverse	`TGGTTGGTCCTCAGGGTTAGA`
ABCG5	NM_031884	Probe	`TGATTGGCAGCTATAATTTTGGG`
		Forward	`CCACGTGGCGGACCAA`
		Reverse	`CGCTCGCCACTGGAAATT`
ABCG8	NM_026180	Probe	`TTTTCCACCCTGATCCGTCG`
		Forward	`GCTGCCCGGGATGATAGAG`
		Reverse	`CCGGAAGTCATTGGAAATCTG`
IL-1β	NM_008361	Probe	`CATGGCACATTCTGTTCAAAGAGAGCCTG`
		Forward	`TCGCTCAGGGTCACAAAGAAA`
		Reverse	`CCATCAGAGGCAAGGAGGAA`
GK	NM_010292	Probe	`AGGACGCCATGACGGGCACT`
		Forward	`GAAGACGAAACACCAGATGTATTC`
		Reverse	`GATGTAGTCAAAGAGCATCTCCG`
Mouse/rat
EF1α	NM_010106	Probe	`TTTGCTGTTCGTGACATGAGGCAG`
		Forward	`GACTACCCTCCACTTGGTCG`
		Reverse	`GATGACACCCACAGCAACTG`