TABLE 1

Blockade of nAChRs does not significantly change burst-stimulated dopamine release in control and lesioned rats

Dopamine release in the absence or presence of α-CtxMII or mecamylamine after a single pulse stimulation or a four pulse stimulus at either 30 or 100 Hz. NAChR inhibition decreased single-pulse stimulated dopamine release while it did not significantly affect burst-induced release regardless of the lesion size. Values represent the mean ± S.E.M. of nine controls and four rats in each lesioned group.

GroupDopamine Release
TotalCtxMIIMecamylamine
One PulseFour PulsesOne PulseFour PulsesOne PulseFour Pulses
30 Hz100 Hz30 Hz100 Hz30 Hz100 Hz
nM
Control317 ± 32388 ± 65408 ± 73175 ± 28261 ± 42393 ± 59**89 ± 9.4207 ± 43*472 ± 45***†
Mild156 ± 31195 ± 47243 ± 4498 ± 24104 ± 21214 ± 7262 ± 12150 ± 30201 ± 43*
Moderate103 ± 19115 ± 24115 ± 2266 ± 1888 ± 20120 ± 2343 ± 1084 ± 17102 ± 23
Moderately severe66 ± 9.265 ± 8.982 ± 1352 ± 1156 ± 14102 ± 3043 ± 9.362 ± 1588 ± 21
Severe6.1 ± 6.17.4 ± 7.46.6 ± 6.66.9 ± 6.96.2 ± 6.27.1 ± 7.17.2 ± 7.24.1 ± 4.19.6 ± 9.6

  • Bonferroni post hoc test was used to calculate significance:

  • ↵* P < 0.05, significantly different from one pulse.

  • ↵** P < 0.01, significantly different from one pulse.

  • ↵*** P < 0.001, significantly different from one pulse.

  • ↵† P < 0.001, significantly different from four pulses at 30 Hz.