TABLE 1

Effect of the inhibition of different MAPK on the level of miRNA-133b, Pitx3, TH, and DAT in zebrafish embryos

Summary of the results obtained by qRT-PCR analysis of miR-133b, Pitx3, TH, and DAT levels in 24-hpf zebrafish embryos exposed to different MAPK inhibitors. The values represent average ± S.E.M. of the miR and mRNA copy numbers determined as described under Materials and Methods.

MAPK Inhibitors	miR-133b	Pitx3	TH	DAT
Control	7.10 × 10⁵ ± 10,260	66,684 ± 3537	25,465 ± 3006	2730 ± 508
JNKII-inhibitor (0.6 μM)	1.73 × 10⁵ ± 9229^**	1.33 × 10⁵ ± 5786^**	50,799 ± 3031^**	11,777 ± 1120^**
SB203580 (60 μM)	2.97 × 10⁵ ± 15,142^**	1.51 × 10⁵ ± 7494^**	52,061 ± 2575^**	18,492 ± 2311^**
PD98059 (50 μM)	1.04 × 10⁶ ± 19,805^*	40,060 ± 2797^*	17,546 ± 771^*	1231 ± 250^*

↵* P ≤ 0.05 (unpaired Student's t test with Welch correction).
↵** P ≤ 0.005 (unpaired Student's t test with Welch correction).